第846回 生医研セミナー(多階層生体防御システム研究拠点)
下記の通り、中條 暖奈 先生によるセミナーを開催いたします。皆様のご来聴を心より歓迎いたします。
※セミナーは日本語で行われます。
演題 / Title
Extracellular matrix remodeling by microglia regulates synapse plasticity in the developing brain
演者 / Speaker
中條 暖奈 先生（Ph.D.）
Postdoctoral Scholar
University of California, San Francisco, CA, USA
日時 / Date
２０２４年１１月２６日（火）
17：00〜18：00
場所 / Venue
病院キャンパス 総合研究棟 1階 講義室 101
以下の地図の34番です。
キャンパスマップ
要 旨 / Abstract
Neuronal connectivity is dynamically remodeled during development through generation and elimination of synapses. Synapse remodeling is robust in early life, but decreases in adulthood, coincident with the accumulation of extracellular matrix (ECM) in the brain, which restricts synaptic plasticity. It was recently showed that microglia, the innate immune cells of the brain, remodel the ECM to promote synapse formation and plasticity in adult mice hippocampus. However, it remains unclear whether microglia interact with ECM in the developing brain and how this impacts synapse dynamics. By taking advantage of zebrafish, we investigated the relationship between microglia, ECM, and synapses in the developing hindbrain, which contains a unique population of synapse-associated microglia as described in a previous study from the lab. By performing time course live imaging of hindbrain motor neurons, we observed that synapses are continuously being formed and removed even though total synapse numbers remained stable in early development. We found that enzymatic ECM digestion decreased the new synapse formation. We also identified a metalloprotease as a microglial-expressed molecule. Deletion of the metalloprotease led to an accumulation of ECM, and an increase in new synapses. Our data revealed a molecular mechanism through which ECM stabilizes newborn synapses during brain development.
参考論文 / References
- Escoubas C. et al. Type-I-interferon-responsive microglia shape cortical development and behavior. Cell 187(8), 2024.
- Shibayama K. and Nakajo H. et al. The serotonergic neurons derived from rhombomere 2 are localized in the median raphe and project to the dorsal pallium in zebrafish. Neuroscience Research 205, 2024.
- Nakajo H. et al. Hunger potentiates the habenular winner pathway for social conflict by orexin-promoted biased alternative splicing of the AMPA receptor gene. Cell Reports 31(12), 2020.
- Nakajo H. et al. The behavioral paradigm to induce repeated social defeats in zebrafish. Neuroscience Research 161, 2020.
連絡先 / Contact
生体防御医学研究所 分子神経免疫学分野
増田 隆博
092 (642) 6800