第684回 生医研セミナー(多階層生体防御システム研究拠点)

下記の通り、Montpellier大学(フランス)のRobert Feil博士によるセミナーを開催致します。
皆様のご来聴を心より歓迎いたします。

演題

Role of non-coding RNA expression in mammalian genomic imprinting.
(seminar in English)

演者

Dr. Robert Feil, Head of Laboratory,
Institute of Molecular Genetics (IGMM), CNRS and the University of Montpellier, France

日時

2014年 11月17日(月) Nov. 17 (Mon), 2014
10:30~11:30

場所

九州大学 病院キャンパス内 生体防御医学研究所 本館1階 会議室
以下の地図の21番の建物になります。
(http://www.kyushu-u.ac.jp/access/map/hospital/hospital.html)

Seminar Room, Main Building 1F, Medical Institute of Bioregulation
No.21 on the following linked map.
(http://www.kyushu-u.ac.jp/access/map/hospital/hospital-e.html)

要旨

Imprinted gene expression in mammals is mediated by differentially methylated CpG islands, called ‘imprinting control regions’ (ICRs). The germ line-acquired DNA methylation imprints at ICRs are maintained throughout development and after birth. This exceptional maintenance process is linked to specific histone modifications and can be recapitulated following ICR insertion into an ectopic locus. Several of the ‘maternal ICRs’ express mono-allelic long non-coding RNAs (ncRNAs) that mediate gene repression in cis through recruitment of Polycomb repressive complexes and the KMT G9a. The Dlk1-Dio3 locus on mouse chromosome-12 is amongst the few imprinted loci controlled by a ‘paternal ICR’. The unmethylated copy of this intergenic ICR activates imprinted gene expression along the domain early in development. This process is linked to mono-allelic expression of ncRNAs from the ICR. Disruption of ICR ncRNA expression in ES cells affects imprinted gene expression in cis, leads to acquisition of aberrant histone and DNA methylation, delays replication timing and changes chromosome configuration. Similarly as in imprinting-related diseases, these epigenetic alterations persist upon differentiation, and affect the cortico-neurogenic potential of the stem cells.

業績

  1. Kota, S.K. et al. ICR non-coding RNA expression controls imprinting and DNA replication at the Dlk1-Dio3 domain. Developmental Cell, (in press).
  2. Girardot, M. et al. PRMT5-mediated histone H4 arginine-3 symmetrical dimethylation marks chromatin at G+C rich regions of the mouse genome. Nucleic Acids Res., 42, 235-248 (2014).
  3. Kelsey, G. and Feil, R. New insights into the establishment and maintenance of DNA methylation imprints in mammals. Phil. Trans. R. Soc. B, 368: 20110336 (2013).

連絡先

生体防御医学研究所 エピゲノム制御学分野 佐々木 裕之
Division of Epigenomics and Development, MIB, Hiroyuki SASAKI
電話:092-642-6759