The 837th MIB Seminar
(Joint Usage/Research Center for the Multi-stratified Host Defense System)
Title
Stem cell potency and cellular identity in the germline
Speaker
Satoshi Namekawa, Ph.D.
Professor
Department of Microbiology & Molecular Genetics, University of California, Davis
Date
Jul. 4 (Tue), 2023
17：30〜
Venue
Seminar Room 105, 1F, Biomedical Research Station, Hospital Campus
No.34 on the map.
Abstract
Spermatogonial stem cells (SSCs) support life-long fertility in adult males by balancing self-renewal anddifferentiation to produce haploid gametes in a slow-cycling cell population. We discover that an epigenetic regulator, Polycomb repressive complex 1 (PRC1), shields SSC potency, maintains slow cycling, and directs commitment o differentiation during steady-state spermatogenesis in adults. Importantly, unlike other tissue-specific stem cells where PRC1 promotes cell cycle progression, PRC1 functions as a negative regulator of the SSC cell cycle that maintains slow cycling. Further, by performing a high-resolution Hi-C analysis of representative stages of mouse spermatogenesis, we found that 3D chromatin predetermines gene expression programs required for spermatogenesis. We propose that CTCF-mediated 3D chromatin underlines epigenetic priming to direct unidirectional differentiation in the male germline.
References
- Alavattam KG, et al. Nat Struct Mol Biol. 2019;26(3):175-84.
- Maezawa S, et al. Nat Struct Mol Biol, 2020; 27(10): 978-988.
- Sakashita A, et al. Nat Struct Mol Biol, 2020; 27 (10): 967-977.
- Hu M, et al. Nat Commun, 2022;13(1):4510.
- Abe H, et al. Nat Commun, 2022;13(1):7212.
Contact
Sasaki Laboratory (Epigenomics and Development), Medical Institute of Bioregulation
Naoki Kubo, M.D., Ph.D. （Research Lecturer）
TEL: 092 (642) 6760