研究実績
2025
1.
Atsushi Takasu, Toshiaki Hino, Osamu Takenouchi, Yasuki Miyagawa, Zhihua Liang, Shota Tanaka, Tomoya Mimura, Chisato Ida, Yuki Matsuo, Yuna Lee, Haruka Ikegami, Miho Ohsugi, Shogo Matoba, Atsuo Ogura, Kazuo Yamagata, Kazuya Matsumoto, Tomoya S Kitajima, Kei Miyamoto
Characterization of H3K4me3 in mouse oocytes at the metaphase II stage Journal Article
In: J Biol Chem, pp. 110308, 2025, ISSN: 1083-351X.
Abstract | Links | タグ: Miyamoto G, Yamagata G
@article{pmid40449591,
title = {Characterization of H3K4me3 in mouse oocytes at the metaphase II stage},
author = {Atsushi Takasu and Toshiaki Hino and Osamu Takenouchi and Yasuki Miyagawa and Zhihua Liang and Shota Tanaka and Tomoya Mimura and Chisato Ida and Yuki Matsuo and Yuna Lee and Haruka Ikegami and Miho Ohsugi and Shogo Matoba and Atsuo Ogura and Kazuo Yamagata and Kazuya Matsumoto and Tomoya S Kitajima and Kei Miyamoto},
doi = {10.1016/j.jbc.2025.110308},
issn = {1083-351X},
year = {2025},
date = {2025-05-29},
urldate = {2025-05-01},
journal = {J Biol Chem},
pages = {110308},
abstract = {Central functions of histone modifications in germ cell and embryonic development have been documented. Accumulating evidence suggests that oocytes possess unique profiles of histone modifications, among which histone H3 lysine 4 trimethylation (H3K4me3) is broadly spread on the mouse oocyte chromosomes at the metaphase II (MII) stage, unlike later embryonic stages. However, the characteristics and developmental roles of H3K4me3 on MII chromosomes are unclear. Here, we discovered that H3K4me3 was abundantly localized on some of the MII oocyte chromosomes facing the cortical side. Using multicolor FISH and CRISPR-Sirius-based labeling of chromosomes, we revealed that the X chromosome tended to be localized at the cortical side with strong H3K4me3 signals. Anchoring oocyte chromosomes to the cortex may play a role in the asymmetric H3K4me3 distribution. Furthermore, we found that the forced removal of H3K4me3 through the overexpression of a specific lysine demethylase in MII oocytes resulted in abnormal chromosome-spindle structure and impaired preimplantation development after in vitro fertilization. These findings highlight the developmental function of H3K4me3 in transcriptionally-silent MII oocytes.},
keywords = {Miyamoto G, Yamagata G},
pubstate = {published},
tppubtype = {article}
}
Central functions of histone modifications in germ cell and embryonic development have been documented. Accumulating evidence suggests that oocytes possess unique profiles of histone modifications, among which histone H3 lysine 4 trimethylation (H3K4me3) is broadly spread on the mouse oocyte chromosomes at the metaphase II (MII) stage, unlike later embryonic stages. However, the characteristics and developmental roles of H3K4me3 on MII chromosomes are unclear. Here, we discovered that H3K4me3 was abundantly localized on some of the MII oocyte chromosomes facing the cortical side. Using multicolor FISH and CRISPR-Sirius-based labeling of chromosomes, we revealed that the X chromosome tended to be localized at the cortical side with strong H3K4me3 signals. Anchoring oocyte chromosomes to the cortex may play a role in the asymmetric H3K4me3 distribution. Furthermore, we found that the forced removal of H3K4me3 through the overexpression of a specific lysine demethylase in MII oocytes resulted in abnormal chromosome-spindle structure and impaired preimplantation development after in vitro fertilization. These findings highlight the developmental function of H3K4me3 in transcriptionally-silent MII oocytes.
