大川計画研究代表、木村計画研究代表、胡桃坂計画研究代表による成果がNature Communications誌に掲載されました!

Reconstructing epigenomic dynamics through a single-cell multi-epigenome data integration framework

Takeru Fujii, Kosuke Tomimatsu, Michiko Kato, Miho Ito, Shoko Sato, Hitoshi Kurumizaka, Yuko Sato, Kazumitsu Maehara, Hiroshi Kimura, Akihito Harada & Yasuyuki Ohkawa

Abstract
Transcriptional regulation arises from the dynamic and combinatorial actions of multiple regulatory factors on genomic DNA. Although many epigenomic regulators have been identified, the precise order in which these factors accumulate at individual gene loci to activate transcription remains unclear. Here we show a single-cell data integration framework that infers the binding order of multiple chromatin factors at single-cell resolution. Central to this framework is sci-mtChIL-seq, a scalable single-cell method that simultaneously profiles genome-wide binding of RNA polymerase II (RNAPII) and diverse epigenomic regulators. By defining transcriptional states through RNAPII occupancy and integrating multiple sci-mtChIL-seq datasets, we systematically link the combinatorial patterns of transcription factor binding, histone modifications and chromatin remodeling. This framework reveals the temporal coordination among chromatin factors during transcriptional activation, providing a powerful approach to uncover context-dependent epigenomic dynamics and the principles of gene regulation in complex cellular systems.

Nature Communications, doi: 10.1038/s41467-025-67016-9. (2025)
https://www.nature.com/articles/s41467-025-67016-9