第 623 回生医研セミナー(多階層生体防御システム研究拠点)
ゲノミクスエピゲノミクス研究拠点セミナー

この度、シンガポール大学の甲斐歳恵先生に生体防御医学研究所でのセミナーをお願い致しました。このセミナーでは、ショウジョウバエの生殖細胞でpiRNAの合成に関わる新規タンパク質kumoについてお話しいただきます。講演は英語で行われます。
また、休憩をはさんで、シンガポールで独立したラボを率いておられる甲斐先生に向こうでの経験談をお願いしました。海外留学を考えている方、海外で独立したい方、お気軽にお越しください。こちらは日本語です。

演題Tudor domain proteins and Piwis function in nuage for piRNA amplification in stem cells and germline cells (16:00〜17:00)
(seminar in English)

海外で独立したラボを持つってどんな感じ?(シンガポール編)(17:10〜18:00)

演者甲斐歳恵 先生  Dr. Toshie KAI (Ph.D)
Senior Principal Investigator/Adjunct Associate Prof.
Temasek Lifescience Laboratories, Department of Biological Science
The National University of Singapore

日時平成24年6月25日(月)  Jun. 25 (Mon), 2012
16:00〜18:00

場所馬出医学系キャンパス内 コラボ・ステーションT 共同セミナー室A・B
以下の地図の32番になります。
http://www.kyushu-u.ac.jp/access/map/hospital/hospital.html

Station-I for Collaborative Research 1F, Seminar Room A/B
No.32 on the following linked map
http://www.kyushu-u.ac.jp/access/map/hospital/hospital-e.html

要旨 The piRNAs provide defense against transposons in animal gonads. In Drosophila ovaries, distinct piRNA pathways function in somatic and germline cells. The germline piRNAs arise from bidirectional clusters and are amplified by ping-pong cycle. We report kumo, a conserved component of the germline piRNA pathway in Drosophila. Kumo is a tudor domain protein that localizes to nuage; a unique structure present in germline cells, proposed as a processing site of germline piRNAs where Piwi family proteins are also localized. kumo is required for localization of all other examined piRNA pathway components to nuage, suggesting that kumo is required as upstream component for nuage organization. kumo is also important for production of sufficient cluster- and ping-pong- derived germline piRNA, but not for somatic piRNAs. In addition to nuage, Kumo also localizes to the nucleus in the germarium and promotes the production of longer precursor transcripts from the germline piRNA clusters. Our data suggest that kumo is important for the production of germline piRNAs by orchestrating organization of nuage and promoting transcription from the germline piRNA clusters in the Drosophila. Interestingly, Piwi proteins are expressed in the multipotent or totipotent stem cells of other animals, implying a wider role of such genes throughout the animal kingdom. I also discuss our recent observation on the expression patterns and possible functions of two Piwi orthologs, Piwi1 and Piwi2, in the basal metazoan, Hydra.

業績
  1. Anand A, Kai T. The tudor domain protein Kumo is required to assemble the nuage and to generate germline piRNAs in Drosophila. EMBO J. 31, 870-882, 2012
  2. Pek JW, Kai T. A Role for Vasa in Regulating Mitotic Chromosome Condensation in Drosophila. Curr. Biol. 21, 39-44, 2011

連絡先生体防御医学研究所 エピゲノム学分野 佐々木 裕之
Division of Epigenomics, MIB, Hiroyuki SASAKI
電話:092-642-6759


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