Bisulfite PCR primers designed to amplify the differentially methylated regions (DMRs) associated with mouse imprinted genes are listed in this table. DNA polymorphisms identified in the amplified regions between C57BL/6 and JF1 mice (similar to the MSM strain derived from Mus musculus molossinus) are also shown in the form of bisulfite-converted sequence. These polymorphisms should be useful if one wants to know the allelic methylation status of the DMRs.
If you want to use the information described in this table∗, please send an e-mail to Prof. Hiroyuki Sasaki (hsasakibioreg.kyushu-u.ac.jp) for permission. When you publish your data which are produced based on the information in this table, do not forget to cite the following paper.
- Kobayashi, H., Suda, C., Abe, T., Kohara, Y., Ikemura, T. & Sasaki, H.
- Bisulfite sequencing and dinucleotide content analysis of 15 imprinted mouse differentially methylated regions (DMRs): paternally methylated DMRs contain less CpGs than maternally methylated DMRs.
- Cytogenet. Genome Res. 113 (Special Issue on Genomic Imprinting), 130-137 (2006).
∗ Table : Bisulfite primer pairs (105 pairs)