Bisulfite PCR primers for mouse imprinted loci

Bisulfite PCR primers designed to amplify the differentially methylated regions (DMRs) associated with mouse imprinted genes are listed in this table. DNA polymorphisms identified in the amplified regions between C57BL/6 and JF1 mice (similar to the MSM strain derived from Mus musculus molossinus) are also shown in the form of bisulfite-converted sequence. These polymorphisms should be useful if one wants to know the allelic methylation status of the DMRs.
If you want to use the information described in this table, please send an e-mail to Prof. Hiroyuki Sasaki ( for permission. When you publish your data which are produced based on the information in this table, do not forget to cite the following paper.

Kobayashi, H., Suda, C., Abe, T., Kohara, Y., Ikemura, T. & Sasaki, H.
Bisulfite sequencing and dinucleotide content analysis of 15 imprinted mouse differentially methylated regions (DMRs): paternally methylated DMRs contain less CpGs than maternally methylated DMRs.
Cytogenet. Genome Res. 113 (Special Issue on Genomic Imprinting), 130-137 (2006).

Table : Bisulfite primer pairs (105 pairs)