{"id":945,"date":"2026-06-12T16:52:57","date_gmt":"2026-06-12T07:52:57","guid":{"rendered":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/?post_type=informationen&#038;p=945"},"modified":"2026-06-12T16:52:57","modified_gmt":"2026-06-12T07:52:57","slug":"the-research-paper-from-the-kurumizaka-lab-has-been-published-in-the-journal-of-biological-chemistry","status":"publish","type":"informationen","link":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/archives\/informationen\/the-research-paper-from-the-kurumizaka-lab-has-been-published-in-the-journal-of-biological-chemistry","title":{"rendered":"The research paper from the Kurumizaka Lab has been published in the Journal of Biological Chemistry!"},"content":{"rendered":"\n<p class=\"wp-block-paragraph\"><strong>Structural basis of RNA polymerase II transcription on the histone H3-H4 octasome<\/strong><\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Ching-Hao Ho, Kaho Nozawa, Mai Nishimura, Masahiro Oi, Tomoya Kujirai, Mitsuo Ogasawara, Hiroshi Ehara, Shun-ichi Sekine, Yoshimasa Takizawa, Hitoshi Kurumizaka<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>Abstract<\/strong><br>The histone H3-H4 octasome is a nucleosome-like particle in which two DNA gyres are wrapped around each histone (H3-H4)2 tetramer disk, forming a clamshell-like configuration. In the present study, we performed in vitro RNA polymerase II (RNAPII) transcription assays with the H3-H4 octasome and found that RNAPII transcribed the H3-H4 octasome more efficiently than the nucleosome. RNAPII paused at only one position, superhelical location (SHL(-4)) in the H3-H4 octasome, in contrast to pausing at the SHL(-5), SHL(-2), and SHL(-1) positions in the nucleosome. Cryo-EM analysis revealed that two (H3-H4)2 tetramer disks are retained when the RNAPII paused at the SHL(-4) position of the H3-H4 octasome. However, when RNAPII reached the SHL(-0.5) position, five base pairs before the dyad position of the H3-H4 octasome, the proximal (H3-H4)2 tetramer was disassembled, but the distal (H3-H4)2 tetramer still remained on the DNA. Therefore, RNAPII efficiently transcribes the H3-H4 octasome by stepwise (H3-H4)2 tetramer disassembly.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong><em>Journal of Biological Chemistry<\/em><\/strong>, 302, 111340. doi: 10.1016\/j.jbc.2026.111340. (2026)<br><a href=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0021925826002103?via%3Dihub\" target=\"_blank\" rel=\"noreferrer noopener\">https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0021925826002103?via%3Dihub<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Structural basis of RNA polymerase II transcription on the histone H3-H4 octasom &#8230; <\/p>\n","protected":false},"author":2,"featured_media":0,"menu_order":0,"template":"","format":"standard","meta":{"footnotes":""},"class_list":["post-945","informationen","type-informationen","status-publish","format-standard","hentry"],"_links":{"self":[{"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/informationen\/945","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/informationen"}],"about":[{"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/types\/informationen"}],"author":[{"embeddable":true,"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/users\/2"}],"version-history":[{"count":1,"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/informationen\/945\/revisions"}],"predecessor-version":[{"id":946,"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/informationen\/945\/revisions\/946"}],"wp:attachment":[{"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/media?parent=945"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}