{"id":877,"date":"2026-02-28T09:42:44","date_gmt":"2026-02-28T00:42:44","guid":{"rendered":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/?post_type=informationen&#038;p=877"},"modified":"2026-02-28T09:42:44","modified_gmt":"2026-02-28T00:42:44","slug":"the-research-paper-from-the-fukaya-lab-has-been-published-in-the-science-advances","status":"publish","type":"informationen","link":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/archives\/informationen\/the-research-paper-from-the-fukaya-lab-has-been-published-in-the-science-advances","title":{"rendered":"The research paper from the Fukaya Lab has been published in the Science Advances!"},"content":{"rendered":"\n<p><strong>Decoding the molecular logic of rapidly evolving ZAD zinc finger proteins in Drosophila<\/strong><\/p>\n\n\n\n<p>Raku Saito, Yusuke Umemura, Shiho Makino, Takashi Fukaya<\/p>\n\n\n\n<p><strong>Abstract<\/strong><br>The zinc finger\u2013associated domain (ZAD)\u2013containing C2H2 zinc finger proteins (ZAD-ZnFs) represent the most abundant class of transcription factors that emerged during insect evolution, yet their molecular diversity and biological functions remain largely unclear. Here, we established a systematic CRISPR-based protein-tagging approach that enables direct, unambiguous comparison of nuclear localization and genome-wide binding profiles of endogenous ZAD-ZnFs in developing Drosophila embryos. Evidence is provided that a subset of ZAD-ZnFs forms nuclear condensates through the stacking of the N-terminal ZAD dimerization surface. Disruption of condensation activity leads to misregulation of genome-wide binding profiles and lethality, underscoring its functional and physiological significance in development. Integrative chromatin immunoprecipitation sequencing and Micro-C analyses reveal that many ZAD-ZnFs colocalize with core insulator proteins such as CCCTC-binding factor and Centrosomal protein 190 kD to control the formation of topological boundaries. We suggest that the diverse molecular functions of ZAD-ZnFs have evolutionarily arisen from their ancestral role as insulator-binding proteins.<\/p>\n\n\n\n<p><strong><em>Science Advances<\/em><\/strong>, doi: 10.1126\/sciadv.ady7568. (2026)<br><a href=\"https:\/\/www.science.org\/doi\/10.1126\/sciadv.ady7568\" target=\"_blank\" rel=\"noreferrer noopener\">https:\/\/www.science.org\/doi\/10.1126\/sciadv.ady7568<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Decoding the molecular logic of rapidly evolving ZAD zinc finger proteins in Dro &#8230; <\/p>\n","protected":false},"author":2,"featured_media":0,"menu_order":0,"template":"","format":"standard","meta":{"footnotes":""},"class_list":["post-877","informationen","type-informationen","status-publish","format-standard","hentry"],"_links":{"self":[{"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/informationen\/877","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/informationen"}],"about":[{"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/types\/informationen"}],"author":[{"embeddable":true,"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/users\/2"}],"version-history":[{"count":1,"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/informationen\/877\/revisions"}],"predecessor-version":[{"id":878,"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/informationen\/877\/revisions\/878"}],"wp:attachment":[{"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/media?parent=877"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}