{"id":816,"date":"2025-12-30T16:15:46","date_gmt":"2025-12-30T07:15:46","guid":{"rendered":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/?post_type=informationen&#038;p=816"},"modified":"2025-12-30T16:16:42","modified_gmt":"2025-12-30T07:16:42","slug":"the-research-paper-from-the-kurumizaka-lab-has-been-published-in-the-iscience","status":"publish","type":"informationen","link":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/archives\/informationen\/the-research-paper-from-the-kurumizaka-lab-has-been-published-in-the-iscience","title":{"rendered":"The research paper from the Kurumizaka Lab has been published in the iScience!"},"content":{"rendered":"\n<p><strong>A method for cryo-EM analysis of eukaryotic nucleosomes reconstituted in bacterial cells<\/strong><\/p>\n\n\n\n<p>Cheng-Han Ho, Yuki Kobayashi, Mitsuo Ogasawara, Yoshimasa Takizawa, Hitoshi Kurumizaka<\/p>\n\n\n\n<p><strong>Abstract<\/strong><br>Conventional methods for preparing nucleosomes are time-consuming and technically demanding. In the present study, we extended the approach of generating nucleosomes in E. coli by the co-expression of all four histones, allowing nucleosomes to be assembled in cells. The bacterially reconstituted nucleosomes can be readily prepared from the E. coli cells and directly subjected to cryo-EM single particle analysis. Using this method, we obtained a 2.56 \u00c5 nucleosome structure that is highly similar to a previously reported nucleosome crystal structure, validating the use of nucleosomes formed in E. coli for cryo-EM analysis. Unexpectedly, we also discovered a non-canonical nucleosome structure, in which two hexasomes are closely packed. This system provides a robust and efficient platform for structural studies of nucleosomes and nucleosome variants, and may facilitate the discovery of chromatin architectures.<\/p>\n\n\n\n<p><strong><em>iScience<\/em><\/strong>, doi: 10.1016\/j.isci.2025.114453. (2025)<br><a href=\"https:\/\/www.cell.com\/iscience\/fulltext\/S2589-0042(25)02714-2\" target=\"_blank\" rel=\"noreferrer noopener\">https:\/\/www.cell.com\/iscience\/fulltext\/S2589-0042(25)02714-2<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>A method for cryo-EM analysis of eukaryotic nucleosomes reconstituted in bacteri &#8230; <\/p>\n","protected":false},"author":2,"featured_media":0,"menu_order":0,"template":"","format":"standard","meta":{"footnotes":""},"class_list":["post-816","informationen","type-informationen","status-publish","format-standard","hentry"],"_links":{"self":[{"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/informationen\/816","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/informationen"}],"about":[{"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/types\/informationen"}],"author":[{"embeddable":true,"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/users\/2"}],"version-history":[{"count":2,"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/informationen\/816\/revisions"}],"predecessor-version":[{"id":818,"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/informationen\/816\/revisions\/818"}],"wp:attachment":[{"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/media?parent=816"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}