{"id":772,"date":"2025-11-12T05:17:49","date_gmt":"2025-11-11T20:17:49","guid":{"rendered":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/?post_type=informationen&#038;p=772"},"modified":"2025-11-12T05:17:49","modified_gmt":"2025-11-11T20:17:49","slug":"the-research-paper-from-the-kurumizaka-lab-has-been-published-in-the-nature-communications","status":"publish","type":"informationen","link":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/archives\/informationen\/the-research-paper-from-the-kurumizaka-lab-has-been-published-in-the-nature-communications","title":{"rendered":"The research paper from the Kurumizaka Lab has been published in the Nature Communications!"},"content":{"rendered":"\n<p><strong>Structural basis of cyclobutane pyrimidine dimer recognition by UV-DDB in the nucleosome<\/strong><\/p>\n\n\n\n<p>Syota Matsumoto, Yoshimasa Takizawa, Mitsuo Ogasawara, Kana Hashimoto, Lumi Negishi, Wenjie Xu, Haruna Tachibana, Junpei Yamamoto, Shigenori Iwai, Kaoru Sugasawa &amp; Hitoshi Kurumizaka<\/p>\n\n\n\n<p><strong>Abstract<\/strong><br>In mammalian global genomic nucleotide excision repair, UV-DDB plays a central role in recognizing DNA lesions, such as 6-4 photoproducts and cyclobutane pyrimidine dimers, within chromatin. In the present study, we perform cryo-electron microscopy analyses coupled with chromatin-immunoprecipitation to reveal that the cellular UV-DDB binds to UV-damaged DNA lesions in a chromatin unit, the nucleosome, at a position approximately 20 base-pairs from the nucleosomal dyad in human cells. An alternative analysis of the in vitro reconstituted UV-DDB-cyclobutane pyrimidine dimer nucleosome structure demonstrates that the DDB2 subunit of UV-DDB specifically recognizes the cyclobutane pyrimidine dimer lesion at this position on the nucleosome. We also determine the structures of UV-DDB bound to DNA lesions at other positions in purified cellular human nucleosomes. These cellular and reconstituted UV-DDB-nucleosome complex structures provide important evidence for understanding the mechanism by which UV lesions in chromatin are recognized and repaired in mammalian cells.<\/p>\n\n\n\n<p><strong><em>Nature Communications<\/em><\/strong>, doi: 10.1038\/s41467-025-65486-5. (2025)<br><a href=\"https:\/\/www.nature.com\/articles\/s41467-025-65486-5\" target=\"_blank\" rel=\"noreferrer noopener\">https:\/\/www.nature.com\/articles\/s41467-025-65486-5<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Structural basis of cyclobutane pyrimidine dimer recognition by UV-DDB in the nu &#8230; <\/p>\n","protected":false},"author":2,"featured_media":0,"menu_order":0,"template":"","format":"standard","meta":{"footnotes":""},"class_list":["post-772","informationen","type-informationen","status-publish","format-standard","hentry"],"_links":{"self":[{"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/informationen\/772","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/informationen"}],"about":[{"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/types\/informationen"}],"author":[{"embeddable":true,"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/users\/2"}],"version-history":[{"count":1,"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/informationen\/772\/revisions"}],"predecessor-version":[{"id":773,"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/informationen\/772\/revisions\/773"}],"wp:attachment":[{"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/media?parent=772"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}