{"id":646,"date":"2025-08-26T09:53:59","date_gmt":"2025-08-26T00:53:59","guid":{"rendered":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/?post_type=informationen&#038;p=646"},"modified":"2025-08-26T09:53:59","modified_gmt":"2025-08-26T00:53:59","slug":"the-research-paper-by-yamagata-lab-has-been-published-in-the-methods-in-molecular-biology","status":"publish","type":"informationen","link":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/archives\/informationen\/the-research-paper-by-yamagata-lab-has-been-published-in-the-methods-in-molecular-biology","title":{"rendered":"The research paper by Yamagata Lab has been published in the Methods in Molecular Biology!"},"content":{"rendered":"\n<p class=\"wp-block-paragraph\"><strong>T4 DNA-Induced Reconstruction of Artificial Nuclei in Living Mouse Oocytes<\/strong><\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Nao Yonezawa, Yasushi Hiraoka, Tokuko Haraguchi &amp; Kazuo Yamagata<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>Abstract<\/strong><br>Fertilization involves a specialized nuclear formation process distinct from that of somatic cells, and reconstitution methods are useful for understanding its underlying mechanisms. Recently, we successfully reconstructed a nuclear-like structure, termed an \u201cartificial nucleus,\u201d in living mouse oocytes by microinjecting solutions of T4 DNA (~166 kbp). This achievement allowed us to identify the physicochemical properties necessary for nuclear formation. In this paper, we describe a method for constructing artificial nuclei using T4 DNA solutions, which will contribute to future studies on the mechanisms of nuclear formation.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong><em>Methods in Molecular Biology<\/em><\/strong>, doi: 10.1007\/978-1-0716-4714-1_13. (2025)<br><a href=\"https:\/\/link.springer.com\/protocol\/10.1007\/978-1-0716-4714-1_13\" target=\"_blank\" rel=\"noreferrer noopener\">https:\/\/link.springer.com\/protocol\/10.1007\/978-1-0716-4714-1_13<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>T4 DNA-Induced Reconstruction of Artificial Nuclei in Living Mouse Oocytes Nao Y &#8230; <\/p>\n","protected":false},"author":2,"featured_media":0,"menu_order":0,"template":"","format":"standard","meta":{"footnotes":""},"class_list":["post-646","informationen","type-informationen","status-publish","format-standard","hentry"],"_links":{"self":[{"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/informationen\/646","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/informationen"}],"about":[{"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/types\/informationen"}],"author":[{"embeddable":true,"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/users\/2"}],"version-history":[{"count":1,"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/informationen\/646\/revisions"}],"predecessor-version":[{"id":647,"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/informationen\/646\/revisions\/647"}],"wp:attachment":[{"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/media?parent=646"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}