{"id":576,"date":"2025-06-15T12:41:55","date_gmt":"2025-06-15T03:41:55","guid":{"rendered":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/?post_type=informationen&#038;p=576"},"modified":"2025-06-15T12:41:55","modified_gmt":"2025-06-15T03:41:55","slug":"a-new-preprint-from-the-fukaya-lab-has-been-posted-on-biorxiv","status":"publish","type":"informationen","link":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/archives\/informationen\/a-new-preprint-from-the-fukaya-lab-has-been-posted-on-biorxiv","title":{"rendered":"A new preprint from the Fukaya Lab has been posted on bioRxiv!"},"content":{"rendered":"\n<p><strong>Decoding the molecular logic of rapidly evolving ZAD zinc-finger proteins in Drosophila<\/strong><\/p>\n\n\n\n<p>Raku Saito, Yusuke Umemura, Shiho Makino, Takashi Fukaya<\/p>\n\n\n\n<p><strong>Abstract<\/strong><br>The zinc-finger associated domain (ZAD)-containing C2H2 zinc-finger proteins (ZAD-ZnFs) represent the most abundant class of transcription factors that emerged during insect evolution, yet their molecular diversity and biological functions remain largely unclear. Here, we established a systematic CRISPR-based protein-tagging approach that enables direct, unambiguous comparison of nuclear localization and genome-wide binding profiles of endogenous ZAD-ZnFs in developing Drosophila embryos. Evidence is provided that a subset of ZAD-ZnFs forms nuclear condensates through the stacking of the N-terminal ZAD dimerization surface. Disruption of condensation activity leads to misregulation of genome-wide binding profiles and lethality, underscoring its functional and physiological significance in development. Importantly, integrative ChIP-seq and Micro-C data analyses reveal that many ZAD-ZnFs colocalize with core insulator proteins such as CTCF and CP190 to strengthen the formation of topological boundaries. We suggest that the diverse molecular functions of ZAD-ZnFs have evolutionally arisen from their ancestral role as insulator-binding proteins.<\/p>\n\n\n\n<p><strong><em>bioRxiv<\/em><\/strong>, doi: 10.1101\/2025.05.21.655257. (2025)<br><a href=\"https:\/\/www.biorxiv.org\/content\/10.1101\/2025.05.21.655257v1\" target=\"_blank\" rel=\"noreferrer noopener\">https:\/\/www.biorxiv.org\/content\/10.1101\/2025.05.21.655257v1<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Decoding the molecular logic of rapidly evolving ZAD zinc-finger proteins in Dro &#8230; <\/p>\n","protected":false},"author":2,"featured_media":0,"menu_order":0,"template":"","format":"standard","meta":{"footnotes":""},"class_list":["post-576","informationen","type-informationen","status-publish","format-standard","hentry"],"_links":{"self":[{"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/informationen\/576","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/informationen"}],"about":[{"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/types\/informationen"}],"author":[{"embeddable":true,"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/users\/2"}],"version-history":[{"count":1,"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/informationen\/576\/revisions"}],"predecessor-version":[{"id":577,"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/informationen\/576\/revisions\/577"}],"wp:attachment":[{"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/media?parent=576"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}