{"id":546,"date":"2025-05-01T11:32:45","date_gmt":"2025-05-01T02:32:45","guid":{"rendered":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/?post_type=informationen&#038;p=546"},"modified":"2025-05-01T11:32:45","modified_gmt":"2025-05-01T02:32:45","slug":"the-research-paper-by-kurumizaka-lab-has-been-published-in-the-methods-in-molecular-biology","status":"publish","type":"informationen","link":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/archives\/informationen\/the-research-paper-by-kurumizaka-lab-has-been-published-in-the-methods-in-molecular-biology","title":{"rendered":"The research paper by Kurumizaka Lab has been published in the Methods in Molecular Biology!"},"content":{"rendered":"\n<p><strong>High-Resolution Cryo-EM Analyses of Nucleosomes<\/strong><\/p>\n\n\n\n<p>Yoshimasa Takizawa, Cheng-Han Ho, Shoko Sato, Radostin Danev &amp; Hitoshi Kurumizaka<\/p>\n\n\n\n<p><strong>Abstract<\/strong><br>The fundamental chromatin unit is the nucleosome, in which approximately 150 base pairs of DNA are bound to the surface of a symmetric histone octamer containing 2 copies each of histones H2A, H2B, H3, and H4. Over the years, numerous structures of nucleosomes have been determined by X-ray crystallography. However, their structural and functional versatility may not have been fully revealed, due to crystal packing effects. Various structures of nucleosomes and their complexes with nucleosome-binding proteins are now being determined by cryo-electron microscopy (cryo-EM) single-particle analysis, allowing the visualization of their structural diversity. In this report, we present a method for high-resolution structural analyses of nucleosomes by cryo-EM and describe the detailed procedures for nucleosome purification, cryo-EM grid preparation, data collection, and data processing. This method can serve as a good starting point for cryo-EM investigations of nucleosomes and their wide range of complexes.<\/p>\n\n\n\n<p><strong><em>Methods in Molecular Biology<\/em><\/strong>, doi: 10.1007\/978-1-0716-4486-7_6. (2025)<br><a href=\"https:\/\/link.springer.com\/protocol\/10.1007\/978-1-0716-4486-7_6\" target=\"_blank\" rel=\"noreferrer noopener\">https:\/\/link.springer.com\/protocol\/10.1007\/978-1-0716-4486-7_6<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>High-Resolution Cryo-EM Analyses of Nucleosomes Yoshimasa Takizawa, Cheng-Han Ho &#8230; <\/p>\n","protected":false},"author":2,"featured_media":0,"menu_order":0,"template":"","format":"standard","meta":{"footnotes":""},"class_list":["post-546","informationen","type-informationen","status-publish","format-standard","hentry"],"_links":{"self":[{"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/informationen\/546","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/informationen"}],"about":[{"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/types\/informationen"}],"author":[{"embeddable":true,"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/users\/2"}],"version-history":[{"count":1,"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/informationen\/546\/revisions"}],"predecessor-version":[{"id":547,"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/informationen\/546\/revisions\/547"}],"wp:attachment":[{"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/media?parent=546"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}