{"id":453,"date":"2025-03-13T03:38:02","date_gmt":"2025-03-12T18:38:02","guid":{"rendered":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/?post_type=informationen&#038;p=453"},"modified":"2025-03-13T03:38:02","modified_gmt":"2025-03-12T18:38:02","slug":"the-research-paper-by-kurumizaka-lab-has-been-published-in-the-science-advances","status":"publish","type":"informationen","link":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/archives\/informationen\/the-research-paper-by-kurumizaka-lab-has-been-published-in-the-science-advances","title":{"rendered":"The research paper by Kurumizaka Lab has been published in the Science Advances!"},"content":{"rendered":"\n<p class=\"wp-block-paragraph\"><strong>Structural insights into promoter-proximal pausing of RNA polymerase II at +1 nucleosome<\/strong><\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Masahiro Naganuma, Tomoya Kujirai, Haruhiko Ehara, Tamami Uejima, Tomoko Ito, Mie Goto, Mari Aoki, Masami Henmi, Sayako Miyamoto-Kohno, Mikako Shirouzu, Hitoshi Kurumizaka, Shun-Ichi Sekine<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>Abstract<\/strong><br>The metazoan transcription elongation complex (EC) of RNA polymerase II (RNAPII) generally stalls between the transcription start site and the first (+1) nucleosome. This promoter-proximal pausing involves negative elongation factor (NELF), 5,6-dichloro-1-\u03b2-d-ribobenzimidazole sensitivity-inducing factor (DSIF), and transcription elongation factor IIS (TFIIS) and is critical for subsequent productive transcription elongation. However, the detailed pausing mechanism and the involvement of the +1 nucleosome remain enigmatic. Here, we report cryo-electron microscopy structures of ECs stalled on nucleosomal DNA. In the absence of TFIIS, the EC is backtracked\/arrested due to conflicts between NELF and the nucleosome. We identified two alternative binding modes of NELF, one of which reveals a critical contact with the downstream DNA through the conserved NELF-E basic helix. Upon binding with TFIIS, the EC progressed to the nucleosome to establish a paused EC with a partially unwrapped nucleosome. This paused EC strongly restricts EC progression further downstream. These structures illuminate the mechanism of RNAPII pausing\/stalling at the +1 nucleosome.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong><em>Science Advances<\/em><\/strong>, doi: 10.1126\/sciadv.adu0577. (2025)<br><a href=\"https:\/\/www.science.org\/doi\/10.1126\/sciadv.adu0577\" target=\"_blank\" rel=\"noreferrer noopener\">https:\/\/www.science.org\/doi\/10.1126\/sciadv.adu0577<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Structural insights into promoter-proximal pausing of RNA polymerase II at +1 nu &#8230; <\/p>\n","protected":false},"author":2,"featured_media":0,"menu_order":0,"template":"","format":"standard","meta":{"footnotes":""},"class_list":["post-453","informationen","type-informationen","status-publish","format-standard","hentry"],"_links":{"self":[{"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/informationen\/453","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/informationen"}],"about":[{"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/types\/informationen"}],"author":[{"embeddable":true,"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/users\/2"}],"version-history":[{"count":1,"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/informationen\/453\/revisions"}],"predecessor-version":[{"id":454,"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/informationen\/453\/revisions\/454"}],"wp:attachment":[{"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/media?parent=453"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}