{"id":442,"date":"2025-02-22T09:09:01","date_gmt":"2025-02-22T00:09:01","guid":{"rendered":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/?post_type=informationen&#038;p=442"},"modified":"2025-02-22T09:15:15","modified_gmt":"2025-02-22T00:15:15","slug":"the-research-paper-from-the-kurumizaka-and-gotoh-labs-has-been-published-in-the-nature-structural-molecular-biology","status":"publish","type":"informationen","link":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/archives\/informationen\/the-research-paper-from-the-kurumizaka-and-gotoh-labs-has-been-published-in-the-nature-structural-molecular-biology","title":{"rendered":"The research paper from the Kurumizaka, Gotoh, and Kimura Labs has been published in the Nature Structural &amp; Molecular Biology!"},"content":{"rendered":"\n<p><strong>Structural insights into how DEK nucleosome binding facilitates H3K27 trimethylation in chromatin<\/strong><\/p>\n\n\n\n<p>Tomoya Kujirai, Kenta Echigoya, Yusuke Kishi, Mai Saeki, Tomoko Ito, Junko Kato, Lumi Negishi, Hiroshi Kimura, Hiroshi Masumoto, Yoshimasa Takizawa, Yukiko Gotoh &amp; Hitoshi Kurumizaka<\/p>\n\n\n\n<p><strong>Abstract<\/strong><br>Structural diversity of the nucleosome affects chromatin conformations and regulates eukaryotic genome functions. Here we identify DEK, whose function is unknown, as a nucleosome-binding protein. In embryonic neural progenitor cells, DEK colocalizes with H3 K27 trimethylation (H3K27me3), the facultative heterochromatin mark. DEK stimulates the methyltransferase activity of Polycomb repressive complex 2 (PRC2), which is responsible for H3K27me3 deposition in vitro. Cryo-electron microscopy structures of the DEK\u2013nucleosome complexes reveal that DEK binds the nucleosome by its tripartite DNA-binding mode on the dyad and linker DNAs and interacts with the nucleosomal acidic patch by its newly identified histone-binding region. The DEK\u2013nucleosome interaction mediates linker DNA reorientation and induces chromatin compaction, which may facilitate PRC2 activation. These findings provide mechanistic insights into chromatin structure-mediated gene regulation by DEK.<\/p>\n\n\n\n<p><strong><em>Nature Structural &amp; Molecular Biology<\/em><\/strong>, doi: 10.1038\/s41594-025-01493-w. (2025)<br><a href=\"https:\/\/www.nature.com\/articles\/s41594-025-01493-w\" target=\"_blank\" rel=\"noreferrer noopener\">https:\/\/www.nature.com\/articles\/s41594-025-01493-w<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Structural insights into how DEK nucleosome binding facilitates H3K27 trimethyla &#8230; <\/p>\n","protected":false},"author":2,"featured_media":0,"menu_order":0,"template":"","format":"standard","meta":{"footnotes":""},"class_list":["post-442","informationen","type-informationen","status-publish","format-standard","hentry"],"_links":{"self":[{"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/informationen\/442","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/informationen"}],"about":[{"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/types\/informationen"}],"author":[{"embeddable":true,"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/users\/2"}],"version-history":[{"count":2,"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/informationen\/442\/revisions"}],"predecessor-version":[{"id":445,"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/informationen\/442\/revisions\/445"}],"wp:attachment":[{"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/media?parent=442"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}