{"id":331,"date":"2024-09-13T04:49:35","date_gmt":"2024-09-12T19:49:35","guid":{"rendered":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/?post_type=informationen&#038;p=331"},"modified":"2024-09-13T04:49:35","modified_gmt":"2024-09-12T19:49:35","slug":"the-research-paper-by-nakayama-lab-has-been-published-in-the-scientific-reports","status":"publish","type":"informationen","link":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/archives\/informationen\/the-research-paper-by-nakayama-lab-has-been-published-in-the-scientific-reports","title":{"rendered":"The research paper by Nakayama Lab has been published in the Scientific Reports!"},"content":{"rendered":"\n<p class=\"wp-block-paragraph\"><strong>Comprehensive posttranslational modifications in the testis-specific histone variant H3t protein validated in tagged knock-in mice<\/strong><\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Takayuki Kawaguchi, Michihiro Hashimoto, Reiko Nakagawa, Ryunosuke Minami, Masahito Ikawa, Jun-ichi Nakayama &amp; Jun Ueda<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>Abstract<\/strong><br>During the development of multicellular organisms and cell differentiation, the chromatin structure in the cell nucleus undergoes extensive changes, and the nucleosome structure is formed by a combination of various histone variants. Histone variants with diverse posttranslational modifications are known to play crucial roles in different regulatory functions. We have previously reported that H3t, a testis-specific histone variant, is essential for spermatogenesis. To elucidate the function of this chromatin molecule in vivo, we generated knock-in mice with a FLAG tag attached to the carboxyl terminus of H3t. In the present study, we evaluated the utility of the generated knock-in mice and comprehensively analyzed posttranslational modifications of canonical H3 and H3t using mass spectrometry. Herein, we found that H3t-FLAG was incorporated into spermatogonia and meiotic cells in the testes, as evidenced by immunostaining of testicular tissue. According to the mass spectrometry analysis, the overall pattern of H3t-FLAG posttranslational modification was comparable to that of the control H3, while the relative abundances of certain specific modifications differed between H3t-FLAG and the control bulk H3. The generated knock-in mice could be valuable for analyzing the function of histone variants in vivo.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong><em>Sci Rep<\/em><\/strong>, doi: 10.1038\/s41598-024-72362-7<br>PMID:<br><a href=\"https:\/\/www.nature.com\/articles\/s41598-024-72362-7\">https:\/\/www.nature.com\/articles\/s41598-024-72362-7<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Comprehensive posttranslational modifications in the testis-specific histone var &#8230; <\/p>\n","protected":false},"author":2,"featured_media":0,"menu_order":0,"template":"","format":"standard","meta":{"footnotes":""},"class_list":["post-331","informationen","type-informationen","status-publish","format-standard","hentry"],"_links":{"self":[{"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/informationen\/331","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/informationen"}],"about":[{"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/types\/informationen"}],"author":[{"embeddable":true,"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/users\/2"}],"version-history":[{"count":1,"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/informationen\/331\/revisions"}],"predecessor-version":[{"id":332,"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/informationen\/331\/revisions\/332"}],"wp:attachment":[{"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/media?parent=331"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}