{"id":1041,"date":"2026-09-29T08:40:09","date_gmt":"2026-09-28T23:40:09","guid":{"rendered":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/?post_type=informationen&#038;p=1041"},"modified":"2026-09-30T09:38:41","modified_gmt":"2026-09-30T00:38:41","slug":"the-research-paper-from-the-kurumizaka-and-ohkawa-labs-has-been-published-in-communications-biology","status":"publish","type":"informationen","link":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/archives\/informationen\/the-research-paper-from-the-kurumizaka-and-ohkawa-labs-has-been-published-in-communications-biology","title":{"rendered":"The research paper from the Kurumizaka and Ohkawa Labs has been published in Communications Biology!"},"content":{"rendered":"\n<p class=\"wp-block-paragraph\"><strong>Cryo-EM structures of CENP-A nucleosomes isolated from human cells<\/strong><\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Eisho Tanida, Suguru Hatazawa, Takumi Oishi, Yuki Kobayashi, Mitsuo Ogasawara, Marta A. Jaskiewicz, Akihito Harada, Takeru Fujii, Yasuyuki Ohkawa, Yoshimasa Takizawa, Hitoshi Kurumizaka<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>Abstract<\/strong><br>Centromeres ensure accurate chromosome segregation, and their identity is specified epigenetically by the histone H3 variant CENP-A. Although the structures of reconstituted CENP-A nucleosomes have provided important insights, how this epigenetic mark is structurally organized within native chromatin remains incompletely understood. Here, we combine cryogenic electron microscopy with chromatin immunoprecipitation (ChIP-CryoEM) to determine the structures of human CENP-A nucleosomes isolated directly from cells without crosslinking or stabilizing agents. We resolved two distinct cellular CENP-A nucleosome structures: a homotypic CENP-A nucleosome and a heterotypic CENP-A\/H3 nucleosome, which have been proposed to represent centromeric and ectopic nucleosomes, respectively. Both cellular CENP-A nucleosomes assemble as histone octamers and wrap DNA in a left-handed superhelix, supporting an octameric model proposed for native CENP-A chromatin. In addition, the hallmark CENP-A-specific structural features previously characterized in vitro, including the shortened CENP-A N-terminal \u03b1-helix and the distinctive RG loop, are preserved in cellular CENP-A nucleosomes. These findings define the structural organization of CENP-A nucleosomes in cellular chromatin.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong><em>Communications Biology<\/em><\/strong>, 9, 1253. doi: 10.1038\/s42003-026-10946-x. (2026)<br><a href=\"https:\/\/www.nature.com\/articles\/s42003-026-10946-x\" target=\"_blank\" rel=\"noreferrer noopener\">https:\/\/www.nature.com\/articles\/s42003-026-10946-x<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Cryo-EM structures of CENP-A nucleosomes isolated from human cells Eisho Tanida, &#8230; <\/p>\n","protected":false},"author":2,"featured_media":0,"menu_order":0,"template":"","format":"standard","meta":{"footnotes":""},"class_list":["post-1041","informationen","type-informationen","status-publish","format-standard","hentry"],"_links":{"self":[{"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/informationen\/1041","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/informationen"}],"about":[{"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/types\/informationen"}],"author":[{"embeddable":true,"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/users\/2"}],"version-history":[{"count":1,"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/informationen\/1041\/revisions"}],"predecessor-version":[{"id":1042,"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/informationen\/1041\/revisions\/1042"}],"wp:attachment":[{"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/media?parent=1041"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}