{"id":875,"date":"2026-02-28T09:42:02","date_gmt":"2026-02-28T00:42:02","guid":{"rendered":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/?post_type=information&#038;p=875"},"modified":"2026-02-28T09:42:02","modified_gmt":"2026-02-28T00:42:02","slug":"%e6%b7%b1%e8%b0%b7%e8%a8%88%e7%94%bb%e7%a0%94%e7%a9%b6%e4%bb%a3%e8%a1%a8%e3%81%ab%e3%82%88%e3%82%8b%e6%88%90%e6%9e%9c%e3%81%8cscience-advances%e8%aa%8c%e3%81%ab%e6%8e%b2%e8%bc%89%e3%81%95%e3%82%8c","status":"publish","type":"information","link":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/archives\/information\/%e6%b7%b1%e8%b0%b7%e8%a8%88%e7%94%bb%e7%a0%94%e7%a9%b6%e4%bb%a3%e8%a1%a8%e3%81%ab%e3%82%88%e3%82%8b%e6%88%90%e6%9e%9c%e3%81%8cscience-advances%e8%aa%8c%e3%81%ab%e6%8e%b2%e8%bc%89%e3%81%95%e3%82%8c","title":{"rendered":"\u6df1\u8c37\u8a08\u753b\u7814\u7a76\u4ee3\u8868\u306b\u3088\u308b\u6210\u679c\u304cScience Advances\u8a8c\u306b\u63b2\u8f09\u3055\u308c\u307e\u3057\u305f!"},"content":{"rendered":"\n<p><strong>Decoding the molecular logic of rapidly evolving ZAD zinc finger proteins in Drosophila<\/strong><\/p>\n\n\n\n<p>Raku Saito, Yusuke Umemura, Shiho Makino, Takashi Fukaya<\/p>\n\n\n\n<p><strong>Abstract<\/strong><br>The zinc finger\u2013associated domain (ZAD)\u2013containing C2H2 zinc finger proteins (ZAD-ZnFs) represent the most abundant class of transcription factors that emerged during insect evolution, yet their molecular diversity and biological functions remain largely unclear. Here, we established a systematic CRISPR-based protein-tagging approach that enables direct, unambiguous comparison of nuclear localization and genome-wide binding profiles of endogenous ZAD-ZnFs in developing Drosophila embryos. Evidence is provided that a subset of ZAD-ZnFs forms nuclear condensates through the stacking of the N-terminal ZAD dimerization surface. Disruption of condensation activity leads to misregulation of genome-wide binding profiles and lethality, underscoring its functional and physiological significance in development. Integrative chromatin immunoprecipitation sequencing and Micro-C analyses reveal that many ZAD-ZnFs colocalize with core insulator proteins such as CCCTC-binding factor and Centrosomal protein 190 kD to control the formation of topological boundaries. We suggest that the diverse molecular functions of ZAD-ZnFs have evolutionarily arisen from their ancestral role as insulator-binding proteins.<\/p>\n\n\n\n<p><strong><em>Science Advances<\/em><\/strong>, doi: 10.1126\/sciadv.ady7568. (2026)<br><a href=\"https:\/\/www.science.org\/doi\/10.1126\/sciadv.ady7568\" target=\"_blank\" rel=\"noreferrer noopener\">https:\/\/www.science.org\/doi\/10.1126\/sciadv.ady7568<\/a><\/p>\n\n\n\n<p><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Decoding the molecular logic of rapidly evolving ZAD zinc finger proteins in Dro &#8230; <\/p>\n","protected":false},"featured_media":0,"menu_order":0,"template":"","format":"standard","meta":{"footnotes":""},"class_list":["post-875","information","type-information","status-publish","format-standard","hentry"],"_links":{"self":[{"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/information\/875","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/information"}],"about":[{"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/types\/information"}],"version-history":[{"count":1,"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/information\/875\/revisions"}],"predecessor-version":[{"id":876,"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/information\/875\/revisions\/876"}],"wp:attachment":[{"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/media?parent=875"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}