{"id":814,"date":"2025-12-30T16:15:04","date_gmt":"2025-12-30T07:15:04","guid":{"rendered":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/?post_type=information&#038;p=814"},"modified":"2025-12-30T16:17:00","modified_gmt":"2025-12-30T07:17:00","slug":"%e8%83%a1%e6%a1%83%e5%9d%82%e8%a8%88%e7%94%bb%e7%a0%94%e7%a9%b6%e4%bb%a3%e8%a1%a8%e3%81%ab%e3%82%88%e3%82%8b%e6%88%90%e6%9e%9c%e3%81%8ciscience%e8%aa%8c%e3%81%ab%e6%8e%b2%e8%bc%89%e3%81%95%e3%82%8c","status":"publish","type":"information","link":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/archives\/information\/%e8%83%a1%e6%a1%83%e5%9d%82%e8%a8%88%e7%94%bb%e7%a0%94%e7%a9%b6%e4%bb%a3%e8%a1%a8%e3%81%ab%e3%82%88%e3%82%8b%e6%88%90%e6%9e%9c%e3%81%8ciscience%e8%aa%8c%e3%81%ab%e6%8e%b2%e8%bc%89%e3%81%95%e3%82%8c","title":{"rendered":"\u80e1\u6843\u5742\u8a08\u753b\u7814\u7a76\u4ee3\u8868\u306b\u3088\u308b\u6210\u679c\u304ciScience\u8a8c\u306b\u63b2\u8f09\u3055\u308c\u307e\u3057\u305f!"},"content":{"rendered":"\n<p><strong>A method for cryo-EM analysis of eukaryotic nucleosomes reconstituted in bacterial cells<\/strong><\/p>\n\n\n\n<p>Cheng-Han Ho, Yuki Kobayashi, Mitsuo Ogasawara, Yoshimasa Takizawa, Hitoshi Kurumizaka<\/p>\n\n\n\n<p><strong>Abstract<\/strong><br>Conventional methods for preparing nucleosomes are time-consuming and technically demanding. In the present study, we extended the approach of generating nucleosomes in E. coli by the co-expression of all four histones, allowing nucleosomes to be assembled in cells. The bacterially reconstituted nucleosomes can be readily prepared from the E. coli cells and directly subjected to cryo-EM single particle analysis. Using this method, we obtained a 2.56 \u00c5 nucleosome structure that is highly similar to a previously reported nucleosome crystal structure, validating the use of nucleosomes formed in E. coli for cryo-EM analysis. Unexpectedly, we also discovered a non-canonical nucleosome structure, in which two hexasomes are closely packed. This system provides a robust and efficient platform for structural studies of nucleosomes and nucleosome variants, and may facilitate the discovery of chromatin architectures.<\/p>\n\n\n\n<p><strong><em>iScience<\/em><\/strong>, doi: 10.1016\/j.isci.2025.114453. (2025)<br><a href=\"https:\/\/www.cell.com\/iscience\/fulltext\/S2589-0042(25)02714-2\" target=\"_blank\" rel=\"noreferrer noopener\">https:\/\/www.cell.com\/iscience\/fulltext\/S2589-0042(25)02714-2<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>A method for cryo-EM analysis of eukaryotic nucleosomes reconstituted in bacteri &#8230; <\/p>\n","protected":false},"featured_media":0,"menu_order":0,"template":"","format":"standard","meta":{"footnotes":""},"class_list":["post-814","information","type-information","status-publish","format-standard","hentry"],"_links":{"self":[{"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/information\/814","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/information"}],"about":[{"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/types\/information"}],"version-history":[{"count":2,"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/information\/814\/revisions"}],"predecessor-version":[{"id":819,"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/information\/814\/revisions\/819"}],"wp:attachment":[{"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/media?parent=814"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}