{"id":650,"date":"2025-08-26T09:56:22","date_gmt":"2025-08-26T00:56:22","guid":{"rendered":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/?post_type=information&#038;p=650"},"modified":"2025-08-26T09:56:22","modified_gmt":"2025-08-26T00:56:22","slug":"%e5%b1%b1%e7%b8%a3%e8%a8%88%e7%94%bb%e7%a0%94%e7%a9%b6%e5%88%86%e6%8b%85%e3%81%ab%e3%82%88%e3%82%8b%e6%88%90%e6%9e%9c%e3%81%8cmethods-in-molecular-biology%e8%aa%8c%e3%81%ab%e6%8e%b2%e8%bc%89%e3%81%95","status":"publish","type":"information","link":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/archives\/information\/%e5%b1%b1%e7%b8%a3%e8%a8%88%e7%94%bb%e7%a0%94%e7%a9%b6%e5%88%86%e6%8b%85%e3%81%ab%e3%82%88%e3%82%8b%e6%88%90%e6%9e%9c%e3%81%8cmethods-in-molecular-biology%e8%aa%8c%e3%81%ab%e6%8e%b2%e8%bc%89%e3%81%95","title":{"rendered":"\u5c71\u7e23\u8a08\u753b\u7814\u7a76\u5206\u62c5\u306b\u3088\u308b\u6210\u679c\u304cMethods in Molecular Biology\u8a8c\u306b\u63b2\u8f09\u3055\u308c\u307e\u3057\u305f!"},"content":{"rendered":"\n<p><strong>T4 DNA-Induced Reconstruction of Artificial Nuclei in Living Mouse Oocytes<\/strong><\/p>\n\n\n\n<p>Nao Yonezawa, Yasushi Hiraoka, Tokuko Haraguchi &amp; Kazuo Yamagata<\/p>\n\n\n\n<p><strong>Abstract<\/strong><br>Fertilization involves a specialized nuclear formation process distinct from that of somatic cells, and reconstitution methods are useful for understanding its underlying mechanisms. Recently, we successfully reconstructed a nuclear-like structure, termed an \u201cartificial nucleus,\u201d in living mouse oocytes by microinjecting solutions of T4 DNA (~166 kbp). This achievement allowed us to identify the physicochemical properties necessary for nuclear formation. In this paper, we describe a method for constructing artificial nuclei using T4 DNA solutions, which will contribute to future studies on the mechanisms of nuclear formation.<\/p>\n\n\n\n<p><strong><em>Methods in Molecular Biology<\/em><\/strong>, doi: 10.1007\/978-1-0716-4714-1_13. (2025)<br><a href=\"https:\/\/link.springer.com\/protocol\/10.1007\/978-1-0716-4714-1_13\" target=\"_blank\" rel=\"noreferrer noopener\">https:\/\/link.springer.com\/protocol\/10.1007\/978-1-0716-4714-1_13<\/a><\/p>\n\n\n\n<p><\/p>\n","protected":false},"excerpt":{"rendered":"<p>T4 DNA-Induced Reconstruction of Artificial Nuclei in Living Mouse Oocytes Nao Y &#8230; <\/p>\n","protected":false},"featured_media":0,"menu_order":0,"template":"","format":"standard","meta":{"footnotes":""},"class_list":["post-650","information","type-information","status-publish","format-standard","hentry"],"_links":{"self":[{"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/information\/650","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/information"}],"about":[{"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/types\/information"}],"version-history":[{"count":1,"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/information\/650\/revisions"}],"predecessor-version":[{"id":651,"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/information\/650\/revisions\/651"}],"wp:attachment":[{"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/media?parent=650"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}