{"id":451,"date":"2025-03-13T03:37:21","date_gmt":"2025-03-12T18:37:21","guid":{"rendered":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/?post_type=information&#038;p=451"},"modified":"2025-03-13T03:37:21","modified_gmt":"2025-03-12T18:37:21","slug":"%e8%83%a1%e6%a1%83%e5%9d%82%e8%a8%88%e7%94%bb%e7%a0%94%e7%a9%b6%e4%bb%a3%e8%a1%a8%e3%81%ab%e3%82%88%e3%82%8b%e6%88%90%e6%9e%9c%e3%81%8cscience-advances-%e8%aa%8c%e3%81%ab%e6%8e%b2%e8%bc%89%e3%81%95","status":"publish","type":"information","link":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/archives\/information\/%e8%83%a1%e6%a1%83%e5%9d%82%e8%a8%88%e7%94%bb%e7%a0%94%e7%a9%b6%e4%bb%a3%e8%a1%a8%e3%81%ab%e3%82%88%e3%82%8b%e6%88%90%e6%9e%9c%e3%81%8cscience-advances-%e8%aa%8c%e3%81%ab%e6%8e%b2%e8%bc%89%e3%81%95","title":{"rendered":"\u80e1\u6843\u5742\u8a08\u753b\u7814\u7a76\u4ee3\u8868\u306b\u3088\u308b\u6210\u679c\u304cScience Advances \u8a8c\u306b\u63b2\u8f09\u3055\u308c\u307e\u3057\u305f!"},"content":{"rendered":"\n<p><strong>Structural insights into promoter-proximal pausing of RNA polymerase II at +1 nucleosome<\/strong><\/p>\n\n\n\n<p>Masahiro Naganuma, Tomoya Kujirai, Haruhiko Ehara, Tamami Uejima, Tomoko Ito, Mie Goto, Mari Aoki, Masami Henmi, Sayako Miyamoto-Kohno, Mikako Shirouzu, Hitoshi Kurumizaka, Shun-Ichi Sekine<\/p>\n\n\n\n<p><strong>Abstract<\/strong><br>The metazoan transcription elongation complex (EC) of RNA polymerase II (RNAPII) generally stalls between the transcription start site and the first (+1) nucleosome. This promoter-proximal pausing involves negative elongation factor (NELF), 5,6-dichloro-1-\u03b2-d-ribobenzimidazole sensitivity-inducing factor (DSIF), and transcription elongation factor IIS (TFIIS) and is critical for subsequent productive transcription elongation. However, the detailed pausing mechanism and the involvement of the +1 nucleosome remain enigmatic. Here, we report cryo-electron microscopy structures of ECs stalled on nucleosomal DNA. In the absence of TFIIS, the EC is backtracked\/arrested due to conflicts between NELF and the nucleosome. We identified two alternative binding modes of NELF, one of which reveals a critical contact with the downstream DNA through the conserved NELF-E basic helix. Upon binding with TFIIS, the EC progressed to the nucleosome to establish a paused EC with a partially unwrapped nucleosome. This paused EC strongly restricts EC progression further downstream. These structures illuminate the mechanism of RNAPII pausing\/stalling at the +1 nucleosome.<\/p>\n\n\n\n<p><strong><em>Science Advances<\/em><\/strong>, doi: 10.1126\/sciadv.adu0577. (2025)<br><a href=\"https:\/\/www.science.org\/doi\/10.1126\/sciadv.adu0577\" target=\"_blank\" rel=\"noreferrer noopener\">https:\/\/www.science.org\/doi\/10.1126\/sciadv.adu0577<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Structural insights into promoter-proximal pausing of RNA polymerase II at +1 nu &#8230; <\/p>\n","protected":false},"featured_media":0,"menu_order":0,"template":"","format":"standard","meta":{"footnotes":""},"class_list":["post-451","information","type-information","status-publish","format-standard","hentry"],"_links":{"self":[{"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/information\/451","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/information"}],"about":[{"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/types\/information"}],"version-history":[{"count":1,"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/information\/451\/revisions"}],"predecessor-version":[{"id":452,"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/information\/451\/revisions\/452"}],"wp:attachment":[{"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/media?parent=451"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}