{"id":329,"date":"2024-09-13T04:48:16","date_gmt":"2024-09-12T19:48:16","guid":{"rendered":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/?post_type=information&p=329"},"modified":"2024-09-13T04:48:16","modified_gmt":"2024-09-12T19:48:16","slug":"%e4%b8%ad%e5%b1%b1%e8%a8%88%e7%94%bb%e7%a0%94%e7%a9%b6%e4%bb%a3%e8%a1%a8%e3%80%81%e3%81%ab%e3%82%88%e3%82%8b%e6%88%90%e6%9e%9c%e3%81%8c-scientific-reports-%e8%aa%8c%e3%81%ab%e6%8e%b2%e8%bc%89%e3%81%95","status":"publish","type":"information","link":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/archives\/information\/%e4%b8%ad%e5%b1%b1%e8%a8%88%e7%94%bb%e7%a0%94%e7%a9%b6%e4%bb%a3%e8%a1%a8%e3%80%81%e3%81%ab%e3%82%88%e3%82%8b%e6%88%90%e6%9e%9c%e3%81%8c-scientific-reports-%e8%aa%8c%e3%81%ab%e6%8e%b2%e8%bc%89%e3%81%95","title":{"rendered":"\u4e2d\u5c71\u8a08\u753b\u7814\u7a76\u4ee3\u8868\u3001\u306b\u3088\u308b\u6210\u679c\u304c Scientific Reports \u8a8c\u306b\u63b2\u8f09\u3055\u308c\u307e\u3057\u305f!"},"content":{"rendered":"\n

Comprehensive posttranslational modifications in the testis-specific histone variant H3t protein validated in tagged knock-in mice<\/strong><\/p>\n\n\n\n

Takayuki Kawaguchi, Michihiro Hashimoto, Reiko Nakagawa, Ryunosuke Minami, Masahito Ikawa, Jun-ichi Nakayama & Jun Ueda<\/p>\n\n\n\n

Abstract<\/strong>
During the development of multicellular organisms and cell differentiation, the chromatin structure in the cell nucleus undergoes extensive changes, and the nucleosome structure is formed by a combination of various histone variants. Histone variants with diverse posttranslational modifications are known to play crucial roles in different regulatory functions. We have previously reported that H3t, a testis-specific histone variant, is essential for spermatogenesis. To elucidate the function of this chromatin molecule in vivo, we generated knock-in mice with a FLAG tag attached to the carboxyl terminus of H3t. In the present study, we evaluated the utility of the generated knock-in mice and comprehensively analyzed posttranslational modifications of canonical H3 and H3t using mass spectrometry. Herein, we found that H3t-FLAG was incorporated into spermatogonia and meiotic cells in the testes, as evidenced by immunostaining of testicular tissue. According to the mass spectrometry analysis, the overall pattern of H3t-FLAG posttranslational modification was comparable to that of the control H3, while the relative abundances of certain specific modifications differed between H3t-FLAG and the control bulk H3. The generated knock-in mice could be valuable for analyzing the function of histone variants in vivo.<\/p>\n\n\n\n

Sci Rep<\/em><\/strong>, doi: 10.1038\/s41598-024-72362-7
PMID:
https:\/\/www.nature.com\/articles\/s41598-024-72362-7<\/a><\/p>\n\n\n\n

<\/p>\n","protected":false},"excerpt":{"rendered":"

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