{"id":1051,"date":"2026-10-14T09:06:40","date_gmt":"2026-10-14T00:06:40","guid":{"rendered":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/?post_type=information&#038;p=1051"},"modified":"2026-10-15T09:10:00","modified_gmt":"2026-10-15T00:10:00","slug":"%e6%9c%a8%e6%9d%91%e8%a8%88%e7%94%bb%e7%a0%94%e7%a9%b6%e4%bb%a3%e8%a1%a8%e3%81%ab%e3%82%88%e3%82%8b%e6%88%90%e6%9e%9c%e3%81%8cthe-embo-journal%e8%aa%8c%e3%81%ab%e6%8e%b2%e8%bc%89%e3%81%95%e3%82%8c","status":"publish","type":"information","link":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/archives\/information\/%e6%9c%a8%e6%9d%91%e8%a8%88%e7%94%bb%e7%a0%94%e7%a9%b6%e4%bb%a3%e8%a1%a8%e3%81%ab%e3%82%88%e3%82%8b%e6%88%90%e6%9e%9c%e3%81%8cthe-embo-journal%e8%aa%8c%e3%81%ab%e6%8e%b2%e8%bc%89%e3%81%95%e3%82%8c","title":{"rendered":"\u6728\u6751\u8a08\u753b\u7814\u7a76\u4ee3\u8868\u306b\u3088\u308b\u6210\u679c\u304cThe EMBO Journal\u8a8c\u306b\u63b2\u8f09\u3055\u308c\u307e\u3057\u305f!"},"content":{"rendered":"\n<p class=\"wp-block-paragraph\"><strong>Proximity of pre-mRNA 3\u2032 end processing and transcription termination is associated with enhanced gene expression<\/strong><\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Agata St\u0119pie\u0144, Deepshika Pulimamidi, Martyna Plens-Ga\u0142\u0105ska, Maxime Lalonde, Magda Kopczy\u0144ska, Klaudia Ostatek, Mariia Kapeliukha, Hiroshi Kimura, Shazia Irshad, Micha\u0142 R Gdula, Stephan Hamperl, Kinga Kamieniarz-Gdula<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>Abstract<\/strong><br>Two key factors required for pre-mRNA 3\u2032 end processing, CPSF73 and PCF11, are linked to poor cancer prognosis, yet their oncogenic roles remain unclear due to their dual function in transcription termination. Using a colorectal cancer (CRC) progression model, we show that primary tumor cells depend on high CPSF73 and PCF11 levels, whereas metastatic cells are less sensitive. This dependency is not explained by alternative polyadenylation (APA) or transcription-replication conflicts. Instead, we identify an uncoupling of 3\u2032 cleavage and transcription termination during CRC progression. Primary tumor cells exhibit a global shift toward proximal termination alongside distal APA usage, while metastatic cells partially revert to normal termination patterns and favor proximal APA. Notably, the distance between 3\u2032 cleavage and termination is nearly halved in primary tumor cells compared with normal cells and becomes particularly short in oncogenes. Reduced cleavage-termination distance correlates with increased gene expression both within and across cell states. These findings identify a previously unrecognized link between transcription termination architecture and gene expression regulation.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong><em>The EMBO Journal<\/em><\/strong>. doi: 10.1038\/s44318-026-00928-4. (2026)<br><a href=\"https:\/\/link.springer.com\/article\/10.1038\/s44318-026-00928-4\" target=\"_blank\" rel=\"noreferrer noopener\">https:\/\/link.springer.com\/article\/10.1038\/s44318-026-00928-4<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Proximity of pre-mRNA 3\u2032 end processing and transcription termination is associa &#8230; <\/p>\n","protected":false},"featured_media":0,"menu_order":0,"template":"","format":"standard","meta":{"footnotes":""},"class_list":["post-1051","information","type-information","status-publish","format-standard","hentry"],"_links":{"self":[{"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/information\/1051","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/information"}],"about":[{"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/types\/information"}],"version-history":[{"count":1,"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/information\/1051\/revisions"}],"predecessor-version":[{"id":1052,"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/information\/1051\/revisions\/1052"}],"wp:attachment":[{"href":"https:\/\/www.bioreg.kyushu-u.ac.jp\/ext\/epicode\/wp-json\/wp\/v2\/media?parent=1051"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}